Hotspot

ndhD-ccsA

SSC · 144319-144525 · 207 bp

Back to Beta

Pi 0.0325
MarkerSeek score 62.00
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
207
variable_sites
10
pi
0.0325
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 CCCGGATTTCGTTCTCTCAC ATGGTTCATTTACATTAACACTT 55.0 26.1 58.4 52.6 304-306 bp 1.0 43.2
ndhD-ccsA_p2 GGTATTGGCATTTACCCGGA ATGGTTCATTTACATTAACACTT 50.0 26.1 57.6 52.6 318-320 bp 1.0 43.0
ndhD-ccsA_p3 AGGTATTGGCATTTACCCGG ATGGTTCATTTACATTAACACTT 50.0 26.1 57.6 52.6 319-321 bp 1.0 43.0
ndhD-ccsA_p4 GGTATTGGCATTTACCCGGA AGTTATGGTTCATTTACATTAACACT 50.0 26.9 57.6 55.4 322-324 bp 1.0 43.0
ndhD-ccsA_p5 AGGTATTGGCATTTACCCGG AGTTATGGTTCATTTACATTAACACT 50.0 26.9 57.6 55.4 323-325 bp 1.0 43.0