Hotspot

trnL-UAA-trnF-GAA

LSC · 71519-71865 · 347 bp

Back to Beta

Pi 0.0211
MarkerSeek score 58.80
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
347
variable_sites
11
pi
0.0211
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 413-414 bp 1.0 77.2
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTGAACTGGTGTAGTGGCAC 50.0 50.0 57.5 58.0 494 bp 0.333333 49.2
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GTGTAGTGGCACGAGGATTT 50.0 50.0 57.5 57.9 486 bp 0.333333 48.8
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TTTCAGTCCTCTGCTCTTGC 50.0 50.0 57.5 57.8 468 bp 0.333333 48.1
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC AGTGGCACGAGGATTTTCAG 50.0 50.0 57.5 58.2 482 bp 0.333333 48.0