Hotspot

psaA-pafI

LSC · 65206-66009 · 804 bp

Back to Beta

Pi 0.0158
MarkerSeek score 60.60
Primer available yes
Label psaA-pafI

Feature Metadata

label_name
psaA-pafI
feature_type
igs
region
LSC
length_bp
804
variable_sites
19
pi
0.0158
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-pafI_p1 TTTTACTTCTGGTTCCGGCG ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.5 58.0 935-942 bp 1.0 77.5
psaA-pafI_p2 TTTTACTTCTGGTTCCGGCG ATTGGTTGAAGATCACGGGG 50.0 50.0 58.5 57.9 869-876 bp 1.0 76.9
psaA-pafI_p3 TTTTACTTCTGGTTCCGGCG CGATCAAGCCGCTGAGTATT 50.0 50.0 58.5 58.1 937-944 bp 1.0 76.7
psaA-pafI_p4 TTTTACTTCTGGTTCCGGCG AGCCGCTGAGTATTGGAAAC 50.0 50.0 58.5 58.3 931-938 bp 1.0 75.7
psaA-pafI_p5 TTTTACTTCTGGTTCCGGCG CAAGCCGCTGAGTATTGGAA 50.0 50.0 58.5 58.3 933-940 bp 1.0 75.7