Hotspot

trnG-GCC-trnM-CAU

LSC · 59893-60074 · 182 bp

Back to Beta

Pi 0.0196
MarkerSeek score 60.80
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
182
variable_sites
5
pi
0.0196
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.93
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 250-261 bp 1.0 83.7
trnG-GCC-trnM-CAU_p2 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 244-255 bp 1.0 83.3
trnG-GCC-trnM-CAU_p3 TCTTTGCCAAGGAGAAGACG TCACGGGTTCAAATCCTGTC 50.0 50.0 57.8 57.8 243-254 bp 1.0 82.7
trnG-GCC-trnM-CAU_p4 TCTTTGCCAAGGAGAAGACG CACGGGTTCAAATCCTGTCT 50.0 50.0 57.8 57.8 242-253 bp 1.0 82.7
trnG-GCC-trnM-CAU_p5 TCTTTGCCAAGGAGAAGACG TGAGGTCACGGGTTCAAATC 50.0 50.0 57.8 57.8 248-259 bp 1.0 82.6