Hotspot

trnT-GGU-psbD

LSC · 54894-56172 · 1279 bp

Back to Beta

Pi 0.0146
MarkerSeek score 41.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1279
variable_sites
28
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAGCACCATGAAGAGCAACA 55.0 45.0 58.1 58.0 1699-1710 bp 1.0 81.0
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG AGCACCATGAAGAGCAACAA 55.0 45.0 58.1 58.0 1698-1709 bp 1.0 81.0
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG AAACTAGGCGCAAAGAACCA 55.0 45.0 58.1 58.0 1859-1870 bp 1.0 80.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG AACTAGGCGCAAAGAACCAA 55.0 45.0 58.1 58.0 1858-1869 bp 1.0 80.8
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG ACCAAAACTAGGCGCAAAGA 55.0 45.0 58.1 58.0 1863-1874 bp 1.0 80.7