Hotspot

trnK-UUU-rps16

LSC · 28474-29047 · 574 bp

Back to Beta

Pi 0.0377
MarkerSeek score 66.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
574
variable_sites
32
pi
0.0377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.2 57.9 698-726 bp 1.0 74.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.2 57.9 697-725 bp 1.0 74.1
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.3 57.9 712-740 bp 1.0 73.2
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGAAACT 50.0 47.6 58.3 57.9 707-735 bp 1.0 73.1
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGAAACT 50.0 47.6 57.5 57.9 684-712 bp 1.0 71.9