Hotspot

trnL-CAA-ycf2

IRb · 16178-16296 · 119 bp

Back to Beta

Pi 0.0448
MarkerSeek score 68.60
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRb
length_bp
119
variable_sites
8
pi
0.0448
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC ATGGCTTTTCCCGGATGAAA 50.0 45.0 58.0 57.8 235 bp 1.0 80.8
trnL-CAA-ycf2_p2 AAATGGTAGACACGCGAGAC TGGCTTTTCCCGGATGAAAT 50.0 45.0 58.0 57.8 234 bp 1.0 80.8
trnL-CAA-ycf2_p3 TGAAATGGTAGACACGCGAG ATGGCTTTTCCCGGATGAAA 50.0 45.0 58.0 57.8 237 bp 1.0 80.8
trnL-CAA-ycf2_p4 TGAAATGGTAGACACGCGAG TGGCTTTTCCCGGATGAAAT 50.0 45.0 58.0 57.8 236 bp 1.0 80.8
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC AAGATGGCTTTTCCCGGATG 50.0 50.0 58.0 58.2 238 bp 1.0 80.8