Hotspot

rpl20

LSC · 71748-72110 · 363 bp

Back to Berlinia

Pi 0.011

MarkerSeek score 48.10

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 4
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	ACCTACTCTACTTTCCCGGAG	GGGGAAAGAATAACTAAAAGAAACGA	52.4	34.6	58.0	57.9	450 bp	1.0	40.7
rpl20_p2	CCTACTCTACTTTCCCGGAGT	GGGGAAAGAATAACTAAAAGAAACGA	52.4	34.6	58.0	57.9	449 bp	1.0	40.7
rpl20_p3	ACCTACTCTACTTTCCCGGA	GGGGAAAGAATAACTAAAAGAAACGA	50.0	34.6	56.8	57.9	450 bp	1.0	40.7
rpl20_p4	ACCTACTCTACTTTCCCGGAG	GGGGAAAGAATAACTAAAAGAAACG	52.4	36.0	58.0	56.7	450 bp	1.0	40.7
rpl20_p5	CCTACTCTACTTTCCCGGAGT	GGGGAAAGAATAACTAAAAGAAACG	52.4	36.0	58.0	56.7	449 bp	1.0	40.7