Hotspot

rpl32-ndhF

SSC · 130352-131363 · 1012 bp

Back to Berlinia

Pi 0.013

MarkerSeek score 53.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1012
variable_sites: 13
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCCGTAGAAAGAGATGTGGC	TAGGGCGTGTGAACCAAAAA	55.0	45.0	58.1	57.9	1635-1642 bp	1.0	80.4
rpl32-ndhF_p2	AAGCTTTTTGAGTTCCCCGT	TAGGGCGTGTGAACCAAAAA	45.0	45.0	57.9	57.9	1650-1657 bp	1.0	80.3
rpl32-ndhF_p3	CCCGTAGAAAGAGATGTGGC	AGCAACTGAACCGGCAAATA	55.0	45.0	58.1	58.1	1844-1851 bp	1.0	79.9
rpl32-ndhF_p4	CCCGTAGAAAGAGATGTGGC	ATTTAGCAACTGAACCGGCA	55.0	45.0	58.1	58.1	1848-1855 bp	1.0	79.9
rpl32-ndhF_p5	CCCGTAGAAAGAGATGTGGC	TAGCAACTGAACCGGCAAAT	55.0	45.0	58.1	58.1	1845-1852 bp	1.0	79.9