Hotspot

ndhI-ndhG

SSC · 123661-124209 · 549 bp

Back to Berlinia

Pi 0.128

MarkerSeek score 97.50

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 549
variable_sites: 67
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 97.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AATCAAATTGCTTTGGGCCG	CCAACGAAAAGGCCGAAAAA	45.0	45.0	57.9	57.8	754-831 bp	1.0	87.0
ndhI-ndhG_p2	AATCAAATTGCTTTGGGCCG	ATCGGTTCAGGCAAATCCAT	45.0	45.0	57.9	57.6	656-733 bp	1.0	86.5
ndhI-ndhG_p3	TTTGGGCCGTTTACCAATGT	CCAACGAAAAGGCCGAAAAA	45.0	45.0	58.3	57.8	743-820 bp	1.0	85.6
ndhI-ndhG_p4	CTTTGGGCCGTTTACCAATG	CCAACGAAAAGGCCGAAAAA	50.0	45.0	57.6	57.8	744-821 bp	1.0	85.0
ndhI-ndhG_p5	CAAATTGCTTTGGGCCGTTT	CCAACGAAAAGGCCGAAAAA	45.0	45.0	58.4	57.8	751-828 bp	1.0	84.6