Hotspot

rps3-rps19

LSC · 87156-87872 · 717 bp

Back to Berlinia

Pi 0.00456

MarkerSeek score 64.90

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 3
pi: 0.00456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	ACCAAGTCTGAAACCGAGTG	GGGACACGCCAAAAACGATA	50.0	50.0	57.8	58.8	734-793 bp	1.0	75.7
rps3-rps19_p2	ACCAAGTCTGAAACCGAGTG	GGACACGCCAAAAACGATAA	50.0	45.0	57.8	57.0	733-792 bp	1.0	74.6
rps3-rps19_p3	CCAAGTCTGAAACCGAGTGG	GGGACACGCCAAAAACGATA	55.0	50.0	58.5	58.8	733-792 bp	1.0	73.7
rps3-rps19_p4	CAAGTCTGAAACCGAGTGGA	GGGACACGCCAAAAACGATA	50.0	50.0	57.5	58.8	732-791 bp	1.0	73.4
rps3-rps19_p5	CCAAGTCTGAAACCGAGTGG	GGACACGCCAAAAACGATAA	55.0	45.0	58.5	57.0	732-791 bp	1.0	72.6