Hotspot

rps11-rpl36

LSC · 82725-83103 · 379 bp

Back to Berlinia

Pi 0.0211

MarkerSeek score 63.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 8
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGCGCATTCTTACGTAAACC	CGTAGGCGAGGACGAATTAT	50.0	50.0	57.9	57.3	510-537 bp	1.0	75.1
rps11-rpl36_p2	CGTCCATCTCTACGTGAACG	CGTAGGCGAGGACGAATTAT	55.0	50.0	58.2	57.3	486-513 bp	1.0	74.2
rps11-rpl36_p3	TTCTACGCGCATTCTTACGT	CGTAGGCGAGGACGAATTAT	45.0	50.0	57.7	57.3	515-542 bp	1.0	73.5
rps11-rpl36_p4	CGCGCATTCTTACGTAAACC	AAGGGCCTCTGTTCGTAAAA	50.0	45.0	57.9	57.1	553-580 bp	1.0	73.0
rps11-rpl36_p5	CGCGCATTCTTACGTAAACC	GCGAAAAATGTCGACTGATCC	50.0	47.6	57.9	58.0	530-557 bp	1.0	72.3