Hotspot

accD-psaI

LSC · 62286-62826 · 541 bp

Back to Berlinia

Pi 0.0135

MarkerSeek score 56.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 7
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	795-818 bp	1.0	82.9
accD-psaI_p2	AGGTTCACAAACGGCTGAAT	CCCGAAGTAACGCTCATCAA	45.0	50.0	58.0	58.0	887-910 bp	1.0	82.8
accD-psaI_p3	AACGCCTACATTGCATTTGC	CCCGAAGTAACGCTCATCAA	45.0	50.0	58.0	58.0	952-975 bp	1.0	82.7
accD-psaI_p4	AACGCCTACATTGCATTTGC	AACGACATCACGATCCCTTG	45.0	50.0	58.0	58.0	1232-1255 bp	1.0	82.1
accD-psaI_p5	AGGTTCACAAACGGCTGAAT	AACGACATCACGATCCCTTG	45.0	50.0	58.0	58.0	1167-1190 bp	1.0	82.1