Hotspot

trnS-UGA-psbZ

LSC · 38311-38688 · 378 bp

Back to Berlinia

Pi 0.0132

MarkerSeek score 59.60

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.0	58.0	501-502 bp	1.0	80.5
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	508-509 bp	1.0	80.2
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	509-510 bp	1.0	80.2
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	516-517 bp	1.0	80.2
trnS-UGA-psbZ_p5	TTCGTCGAGCAAAAGGAGAG	CGACCAACCATCAGGAGAAG	50.0	55.0	57.9	58.0	586-587 bp	1.0	80.0