Hotspot

trnC-GCA-petN

LSC · 29027-30082 · 1056 bp

Back to Berlinia

Pi 0.00583

MarkerSeek score 63.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 6
pi: 0.00583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1091-1112 bp	1.0	80.5
trnC-GCA-petN_p2	TTTATCGTTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.7	58.2	1144-1165 bp	1.0	78.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1142-1163 bp	1.0	78.6
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1109-1130 bp	1.0	78.3
trnC-GCA-petN_p5	TTTATCGTTTTGGCGGCATG	TCTTCCCCATACTACGAGGG	45.0	55.0	57.7	57.6	1195-1216 bp	1.0	77.0