Hotspot

ycf1

SSC · 126084-131621 · 5538 bp

Back to Bergenia

Pi 0.00813

MarkerSeek score 44.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 67
pi: 0.00813
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTGTATTGATCCGAGGA	AGAACAAGCCTAATCAGAACTCA	42.9	39.1	56.5	57.5	266 bp	1.0	42.7
ycf1_p2	AAGTTGTGTATTGATCCGAGGA	AGAACAAGCCTAATCAGAACTCA	40.9	39.1	57.2	57.5	267 bp	1.0	42.7
ycf1_p3	AGTTGTGTATTGATCCGAGGAA	AGAACAAGCCTAATCAGAACTCA	40.9	39.1	57.2	57.5	266 bp	1.0	42.7
ycf1_p4	AGTTGTGTATTGATCCGAGGA	GAACAAGCCTAATCAGAACTCA	42.9	40.9	56.5	56.1	265 bp	1.0	42.7
ycf1_p5	AGTTGTGTATTGATCCGAGGA	ACAAGCCTAATCAGAACTCA	42.9	40.0	56.5	54.0	263 bp	1.0	42.7