Hotspot

ccsA-ndhD

SSC · 117428-117731 · 304 bp

Back to Bergenia

Pi 0.0322

MarkerSeek score 62.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 304
variable_sites: 14
pi: 0.0322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGTTATGGTTCATTTAACATCTAA	ACCCGGATTTCGTTCTTTCA	25.0	45.0	52.2	57.4	334-396 bp	1.0	47.0
ccsA-ndhD_p2	AGTTATGGTTCATTTAACATCTAA	ACCCGGATTTCGTTCTTTCAT	25.0	42.9	52.2	57.9	334-396 bp	1.0	47.0
ccsA-ndhD_p3	AGTTATGGTTCATTTAACATCTAA	AGGGATTGGTATTTACCCGGA	25.0	47.6	52.2	58.2	348-410 bp	1.0	46.9
ccsA-ndhD_p4	AGTTATGGTTCATTTAACATCTAA	GGGATTGGTATTTACCCGGA	25.0	50.0	52.2	56.7	347-409 bp	1.0	46.9
ccsA-ndhD_p5	AGTTATGGTTCATTTAACATCTAA	AGGGATTGGTATTTACCCGG	25.0	50.0	52.2	56.7	348-410 bp	1.0	46.9