Hotspot

ndhF-rpl32

SSC · 114559-115675 · 1117 bp

Back to Bergenia

Pi 0.0114

MarkerSeek score 56.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1117
variable_sites: 19
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	TTTGACATAGAAGTACGTTTTT	50.0	27.3	57.7	52.0	1203-1213 bp	1.0	42.3
ndhF-rpl32_p2	CTGGAAGTGGAACGAAAGGT	TTTTGACATAGAAGTACGTTTT	50.0	27.3	57.7	52.0	1204-1214 bp	1.0	42.3
ndhF-rpl32_p3	TTTGTTGCCGTCGGAAAAAG	TTTGACATAGAAGTACGTTTTT	45.0	27.3	57.8	52.0	1252-1262 bp	1.0	42.2
ndhF-rpl32_p4	TTTGTTGCCGTCGGAAAAAG	TTTTGACATAGAAGTACGTTTT	45.0	27.3	57.8	52.0	1253-1263 bp	1.0	42.2
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TTTTTGACATAGAAGTACGTTT	45.0	27.3	57.8	52.0	1254-1264 bp	1.0	42.2