Hotspot

petB-petD

LSC · 78986-79826 · 841 bp

Back to Bergenia

Pi 0.00396

MarkerSeek score 64.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 5
pi: 0.00396
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	ACCCCTTCTTACTGCCGTAT	GGCCCATACCTTTAGCCAAT	50.0	50.0	58.1	57.6	1025-1035 bp	1.0	77.9
petB-petD_p2	TTACCCCTTCTTACTGCCGT	GGCCCATACCTTTAGCCAAT	50.0	50.0	58.4	57.6	1027-1037 bp	1.0	76.1
petB-petD_p3	CAGGGTATTTCTGGGCCTTT	GGCCCATACCTTTAGCCAAT	50.0	50.0	57.5	57.6	973-983 bp	1.0	74.7
petB-petD_p4	TACCCCTTCTTACTGCCGTA	GGCCCATACCTTTAGCCAAT	50.0	50.0	57.5	57.6	1026-1036 bp	1.0	74.6
petB-petD_p5	ACCCCTTCTTACTGCCGTAT	CGAAGGAACCGGACATGATA	50.0	50.0	58.1	57.1	929-939 bp	1.0	74.1