Hotspot

trnR-UCU-atpA

LSC · 10606-10840 · 235 bp

Back to Bergenia

Pi 0.0426

MarkerSeek score 69.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 12
pi: 0.0426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCGGAATGAAAAGCGTCCA	GTTTTCTACTTCAGGAACAAGCA	45.0	39.1	57.9	57.2	320-361 bp	1.0	55.9
trnR-UCU-atpA_p2	TCGGAATGAAAAGCGTCCAT	GTTTTCTACTTCAGGAACAAGCA	45.0	39.1	57.9	57.2	319-360 bp	1.0	55.9
trnR-UCU-atpA_p3	ATCGGAATGAAAAGCGTCCA	TTCTACTTCAGGAACAAGCA	45.0	40.0	57.9	54.2	317-358 bp	1.0	55.7
trnR-UCU-atpA_p4	TCGGAATGAAAAGCGTCCAT	TTCTACTTCAGGAACAAGCA	45.0	40.0	57.9	54.2	316-357 bp	1.0	55.7
trnR-UCU-atpA_p5	TCGGAATGAAAAGCGTCCAT	TTTCTACTTCAGGAACAAGCA	45.0	38.1	57.9	55.1	317-358 bp	1.0	54.6