Hotspot

ycf1

IRb · 114068-115141 · 1074 bp

Back to Berchemiella

Pi 0.00186

MarkerSeek score 60.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1074
variable_sites: 3
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAGATGTCGTTTCTAGTCT	AGGGAGTGGTCGAATTTCTTC	40.0	47.6	52.9	57.7	1279-1287 bp	1.0	40.7
ycf1_p2	AGAGATGTCGTTTCTAGTCT	GGAGTGGTCGAATTTCTTCCT	40.0	47.6	52.9	57.7	1277-1285 bp	1.0	40.7
ycf1_p3	AAGAGATGTCGTTTCTAGTCT	AGGGAGTGGTCGAATTTCTTC	38.1	47.6	53.8	57.7	1280-1288 bp	1.0	40.7
ycf1_p4	AAGAGATGTCGTTTCTAGTCT	GGAGTGGTCGAATTTCTTCCT	38.1	47.6	53.8	57.7	1278-1286 bp	1.0	40.7
ycf1_p5	AGAGATGTCGTTTCTAGTCT	AGGGAGTGGTCGAATTTCTT	40.0	45.0	52.9	56.5	1279-1287 bp	1.0	40.7