Hotspot

rpoA

LSC · 82390-83385 · 996 bp

Back to Berchemiella

Pi 0.00134

MarkerSeek score 45.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GTTCTTTCGGGGGATGGATC	TGGCTGTAGACCCCCTAAAA	55.0	50.0	58.0	58.0	1822-1823 bp	1.0	79.8
rpoA_p2	AGCTAAGTTGGCTAAGGGGA	TGGCTGTAGACCCCCTAAAA	50.0	50.0	58.0	58.0	1743-1744 bp	1.0	79.8
rpoA_p3	AGCCCTTTGGTTGGGTATTG	TGGCTGTAGACCCCCTAAAA	50.0	50.0	58.1	58.0	1371-1372 bp	1.0	79.7
rpoA_p4	CAGCCCTTTGGTTGGGTATT	TGGCTGTAGACCCCCTAAAA	50.0	50.0	58.1	58.0	1372-1373 bp	1.0	79.7
rpoA_p5	GTTCTTTCGGGGGATGGATC	TTGGTTCACGTAAAAACGCG	55.0	45.0	58.0	57.9	2159-2160 bp	1.0	79.4