Hotspot

ndhF-rpl32

SSC · 117407-117823 · 417 bp

Back to Berchemiella

Pi 0.00959

MarkerSeek score 61.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 417
variable_sites: 6
pi: 0.00959
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTAACAAGGGGGTTGGAAGG	TGCTACCCAATATCCCTTCCT	55.0	47.6	57.8	57.9	563-565 bp	1.0	71.0
ndhF-rpl32_p2	AGTGCCGTCGGAAAAAGTAG	TGCTACCCAATATCCCTTCCT	50.0	47.6	58.2	57.9	597-599 bp	1.0	70.9
ndhF-rpl32_p3	GTGCCGTCGGAAAAAGTAGA	TGCTACCCAATATCCCTTCCT	50.0	47.6	58.2	57.9	596-598 bp	1.0	70.9
ndhF-rpl32_p4	TTAGTGCCGTCGGAAAAAGT	TGCTACCCAATATCCCTTCCT	45.0	47.6	57.8	57.9	599-601 bp	1.0	70.7
ndhF-rpl32_p5	GGGTTGGAAGGGGAATGAAA	TGCTACCCAATATCCCTTCCT	50.0	47.6	57.7	57.9	554-556 bp	1.0	70.1