Hotspot

atpH-atpI

LSC · 14991-16167 · 1177 bp

Back to Berchemiella

Pi 0.00283

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 5
pi: 0.00283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1230-1236 bp	1.0	80.3
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	GCGAATCCATGGAAGGTCAT	55.0	50.0	58.0	58.0	1315-1321 bp	1.0	80.2
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CCTGTCATGTTCCTTGGCTT	45.0	50.0	58.0	58.1	1306-1312 bp	1.0	79.7
atpH-atpI_p4	GATCCCTTCTACAGCTTGGC	CCTGTCATGTTCCTTGGCTT	55.0	50.0	58.0	58.1	1391-1397 bp	1.0	79.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	AACTTTGGCTGCGGCTTATA	45.0	45.0	58.0	57.9	1253-1259 bp	1.0	79.5