Hotspot

ycf1

SSC · 129673-135429 · 5757 bp

Back to Berchemia

Pi 0.0825

MarkerSeek score 76.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5757
variable_sites: 1160
pi: 0.0825
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCCCACGGTTTAATCTGAT	ACCCCCAGCCCTTAACTTAT	50.0	50.0	57.9	58.0	970-985 bp	0.8	72.5
ycf1_p2	GGTATGGCCCACGGTTTAAT	ACCCCCAGCCCTTAACTTAT	50.0	50.0	57.9	58.0	975-990 bp	0.8	72.5
ycf1_p3	TCCAGGCCTCAATATCGACT	ACCCCCAGCCCTTAACTTAT	50.0	50.0	57.9	58.0	683-698 bp	0.8	72.4
ycf1_p4	TTCGTCCGAGTCTTACCAGT	ACCCCCAGCCCTTAACTTAT	50.0	50.0	58.1	58.0	744-759 bp	0.8	72.2
ycf1_p5	GGCCCACGGTTTAATCTGAT	ACCCCCGAAATTCTGGAATG	50.0	50.0	57.9	57.9	836-851 bp	0.8	71.5