Hotspot

ndhA

SSC · 125499-127767 · 2269 bp

Back to Berchemia

Pi 0.07

MarkerSeek score 82.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2269
variable_sites: 387
pi: 0.07
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACACGAACGCATACTTCACA	CAGGCTCTGGAAGGAATTCC	45.0	55.0	57.9	58.0	2893-2919 bp	1.0	90.5
ndhA_p2	CGAACGCATACTTCACAAGC	CAGGCTCTGGAAGGAATTCC	50.0	55.0	57.9	58.0	2890-2916 bp	1.0	90.4
ndhA_p3	CTCGTACCGTTTGTTGACCA	CAGGCTCTGGAAGGAATTCC	50.0	55.0	58.1	58.0	2727-2753 bp	0.8	71.6
ndhA_p4	ACACGAACGCATACTTCACA	AGCCCCAAAAGGAGAATTGG	45.0	50.0	57.9	58.1	2729-2755 bp	0.8	71.6
ndhA_p5	CTCGTACCGTTTGTTGACCA	AGCCCCAAAAGGAGAATTGG	50.0	50.0	58.1	58.1	2563-2589 bp	0.8	71.6