Hotspot

clpP

LSC · 74684-76778 · 2095 bp

Back to Berchemia

Pi 0.0596

MarkerSeek score 83.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2095
variable_sites: 306
pi: 0.0596
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGTACTGATCGCCGATACT	GGACTATCTCGGGGAAAGGA	50.0	55.0	58.0	57.9	2881-2888 bp	0.8	72.0
clpP_p2	CTGAACGAGTCGCACATACA	GGACTATCTCGGGGAAAGGA	50.0	55.0	58.0	57.9	2823-2830 bp	0.8	71.9
clpP_p3	ATCGATCCTAACCGGACGAT	CGTATAGAGCCATCGAACCG	50.0	55.0	58.1	58.0	2872-2879 bp	0.8	71.8
clpP_p4	CTGAACGAGTCGCACATACA	CTCGGGGAAAGGATTCGTTT	50.0	50.0	58.0	57.9	2816-2823 bp	0.6	63.5
clpP_p5	TGGTACTGATCGCCGATACT	CTCGGGGAAAGGATTCGTTT	50.0	50.0	58.0	57.9	2874-2881 bp	0.6	63.5