Hotspot

trnF-GAA-ndhJ

LSC · 52458-53213 · 756 bp

Back to Berchemia

Pi 0.0713

MarkerSeek score 84.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 133
pi: 0.0713
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTTTATGAAATACAAGATGCTCA	55.0	26.1	58.0	52.4	600-863 bp	1.0	52.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTTTATGAAATACAAGATGCTCA	50.0	26.1	58.1	52.4	564-827 bp	1.0	52.0
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TTTTATGAAATACAAGATGCTCA	55.0	26.1	57.9	52.4	588-851 bp	1.0	52.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	TTTTTATGAAATACAAGATGCTCA	55.0	25.0	58.0	53.3	601-864 bp	1.0	52.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATGAAATACAAGATGCTCATTAA	55.0	26.1	58.0	52.2	849-859 bp	0.8	32.9