Hotspot

trnT-GGU-psbD

LSC · 34423-35572 · 1150 bp

Back to Berchemia

Pi 0.0785

MarkerSeek score 84.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 218
pi: 0.0785
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AGGGTAGGACTTTTCGGTGA	CCAGACTGACCTAGCGGATA	50.0	55.0	58.0	58.0	2337-2413 bp	0.8	72.6
trnT-GGU-psbD_p2	AGGGTAGGACTTTTCGGTGA	AGGTCGCAATTGGACAGATC	50.0	50.0	58.0	58.0	2260-2336 bp	0.8	72.5
trnT-GGU-psbD_p3	AGGGTAGGACTTTTCGGTGA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.0	58.0	1909-1985 bp	0.8	72.5
trnT-GGU-psbD_p4	AGGGTAGGACTTTTCGGTGA	CTTCAGGACCCCATAGCAAC	50.0	55.0	58.0	58.0	2129-2205 bp	0.8	72.4
trnT-GGU-psbD_p5	AGGGTAGGACTTTTCGGTGA	ACAAAAGTCCACAGACCACC	50.0	50.0	58.0	57.9	2181-2257 bp	0.8	72.3