Hotspot

ycf2-trnI-CAU

IRa · 152515-152799 · 285 bp

Back to Berberis

Pi 0.0135

MarkerSeek score 57.40

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 285
variable_sites: 11
pi: 0.0135
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	ATAGACGTCGTTTTGCCCTG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.3	58.0	340-517 bp	1.0	84.2
ycf2-trnI-CAU_p2	ATAGACGTCGTTTTGCCCTG	AGTTGGGCGCTTTAACCATT	50.0	45.0	58.3	58.4	341-518 bp	1.0	81.3
ycf2-trnI-CAU_p3	ATAGACGTCGTTTTGCCCTG	TTGGGCGCTTTAACCATTCA	50.0	45.0	58.3	58.4	339-516 bp	1.0	81.3
ycf2-trnI-CAU_p4	ATAGACGTCGTTTTGCCCTG	CCAATTATGAGTTGGGCGCT	50.0	50.0	58.3	58.6	350-527 bp	1.0	79.4
ycf2-trnI-CAU_p5	ATAGACGTCGTTTTGCCCTG	GAGTTGGGCGCTTTAACCAT	50.0	50.0	58.3	58.8	342-519 bp	1.0	77.7