Hotspot

rps15-trnN-GUU

SSC · 124702-131583 · 6882 bp

Back to Berberis

Pi 0.00948
MarkerSeek score 50.90
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6882
variable_sites
222
pi
0.00948
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.95
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 ACGTATTTTGATTTGCGGGA TTGCTTGACGAGTTCGAAGT 40.0 45.0 56.0 57.8 173-251 bp 1.0 74.6
rps15-trnN-GUU_p2 ACGTATTTTGATTTGCGGGA CGAAGTGGAAGAACGACTGA 40.0 50.0 56.0 57.6 159-237 bp 1.0 73.0
rps15-trnN-GUU_p3 ACGTATTTTGATTTGCGGGA CTTGACGAGTTCGAAGTGGA 40.0 50.0 56.0 57.6 170-248 bp 1.0 73.0
rps15-trnN-GUU_p4 ACGTATTTTGATTTGCGGGA TCGAAGTGGAAGAACGACTG 40.0 50.0 56.0 57.6 160-238 bp 1.0 73.0
rps15-trnN-GUU_p5 ACGTATTTTGATTTGCGGGA TGACGAGTTCGAAGTGGAAG 40.0 50.0 56.0 57.6 168-246 bp 1.0 73.0