Hotspot

ccsA-ndhD

SSC · 116463-116702 · 240 bp

Back to Berberis

Pi 0.0398

MarkerSeek score 79.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 26
pi: 0.0398
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCAATTGTAGCTTCTATGGGA	TGCACTATCAGTTGACAAGGT	40.9	42.9	57.5	57.2	403-434 bp	1.0	53.8
ccsA-ndhD_p2	TGCAATTGTAGCTTCTATGGGA	TCCGGATTTTGTTTTTGCACT	40.9	38.1	57.5	57.2	418-449 bp	1.0	53.2
ccsA-ndhD_p3	TGCAATTGTAGCTTCTATGGGA	CGATCTGTATCCTTCTACCCG	40.9	52.4	57.5	57.2	458-489 bp	1.0	52.4
ccsA-ndhD_p4	TGCAATTGTAGCTTCTATGGGA	TTGCACTATCAGTTGACAAGGT	40.9	40.9	57.5	57.9	404-435 bp	1.0	51.3
ccsA-ndhD_p5	CTGCAATTGTAGCTTCTATGGG	TGCACTATCAGTTGACAAGGT	45.5	42.9	57.2	57.2	404-435 bp	1.0	51.2