Hotspot

rpl32-trnL-UAG

SSC · 114794-115321 · 528 bp

Back to Berberis

Pi 0.013
MarkerSeek score 56.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
528
variable_sites
26
pi
0.013
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTGCGCGACAAACAAGTAA AGCGTGTCTACCGATTTCAC 40.0 50.0 57.2 58.0 543-603 bp 1.0 81.0
rpl32-trnL-UAG_p2 TTTGCGCGACAAACAAGTAA CAGCGTGTCTACCGATTTCA 40.0 50.0 57.2 58.0 544-604 bp 1.0 81.0
rpl32-trnL-UAG_p3 TTTGCGCGACAAACAAGTAA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.2 57.9 553-613 bp 1.0 80.0
rpl32-trnL-UAG_p4 TTTGCGCGACAAACAAGTAA CCTAAGAGCAGCGTGTCTAC 40.0 55.0 57.2 57.8 552-612 bp 1.0 79.4
rpl32-trnL-UAG_p5 TTTGCGCGACAAACAAGTAA CTAAGAGCAGCGTGTCTACC 40.0 55.0 57.2 57.8 551-611 bp 1.0 79.4