Hotspot

ndhF-rpl32

SSC · 113608-114634 · 1027 bp

Back to Berberis

Pi 0.0138

MarkerSeek score 56.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1027
variable_sites: 48
pi: 0.0138
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAGTGGAACCCAAGGTA	CGTTTCTTTGGAACTGCC	50.0	50.0	58.2	54.3	995-1104 bp	1.0	47.6
ndhF-rpl32_p2	GCTGTAGGGACAACGAGAAG	CGTTTCTTTGGAACTGCC	55.0	50.0	58.0	54.3	1039-1148 bp	1.0	47.5
ndhF-rpl32_p3	CGACGAAGACTTTTTGCTGC	CGTTTCTTTGGAACTGCC	50.0	50.0	58.3	54.3	1057-1166 bp	1.0	47.5
ndhF-rpl32_p4	TTTTGCTGCTGTAGGGACAA	CGTTTCTTTGGAACTGCC	45.0	50.0	57.6	54.3	1046-1155 bp	1.0	47.4
ndhF-rpl32_p5	TTTTTGCTGCTGTAGGGACA	CGTTTCTTTGGAACTGCC	45.0	50.0	57.6	54.3	1047-1156 bp	1.0	47.4