Hotspot

trnN-GUU-ndhF

SSC · 108653-111372 · 2720 bp

Back to Berberis

Pi 0.0228

MarkerSeek score 58.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 2720
variable_sites: 169
pi: 0.0228
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	AAATATATAGGGCGCGGACG	50.0	50.0	58.2	57.7	2232-2900 bp	0.941176	83.3
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	GCGCGGACGAATTTCTTTTT	50.0	45.0	58.2	58.3	2221-2889 bp	0.941176	83.1
trnN-GUU-ndhF_p3	GGAACGGGTTACCTACTCCT	GCGCGGACGAATTTCTTTTT	55.0	45.0	58.1	58.3	2323-2991 bp	0.941176	83.0
trnN-GUU-ndhF_p4	CGGGCCCTTTCATTCTGAAT	AAATATATAGGGCGCGGACG	50.0	50.0	58.2	57.7	2303-2971 bp	0.941176	82.5
trnN-GUU-ndhF_p5	GGAACGGGTTACCTACTCCT	AAATATATAGGGCGCGGACG	55.0	50.0	58.1	57.7	2334-2983 bp	0.882353	80.8