Hotspot

rbcL-psaI

LSC · 58360-61282 · 2923 bp

Back to Berberis

Pi 0.0255

MarkerSeek score 66.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 2923
variable_sites: 219
pi: 0.0255
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	ATTTGAATTTGAAGCAATGGA	AGAGGGAAAGTTGAAAATTGTCA	28.6	34.8	52.5	56.5	2652-2978 bp	0.882353	45.2
rbcL-psaI_p2	TTTGAATTTGAAGCAATGGAT	AGAGGGAAAGTTGAAAATTGTCA	28.6	34.8	52.5	56.5	2651-2977 bp	0.882353	45.2
rbcL-psaI_p3	TCTGGAAGGAAATCAAATTTGA	AGAGGGAAAGTTGAAAATTGTCA	31.8	34.8	53.9	56.5	2668-2994 bp	0.882353	45.1
rbcL-psaI_p4	TCTGGAAGGAAATCAAATTTGAA	AGAGGGAAAGTTGAAAATTGTCA	30.4	34.8	54.7	56.5	2668-2994 bp	0.882353	45.1
rbcL-psaI_p5	TCTGGAAGGAAATCAAATTTGA	AAGAGGGAAAGTTGAAAATTGTCA	31.8	33.3	53.9	57.2	2669-2995 bp	0.882353	45.1