Hotspot

trnK-UUU-rps16

LSC · 4390-4964 · 575 bp

Back to Berberis

Pi 0.0122
MarkerSeek score 65.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
575
variable_sites
24
pi
0.0122
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTACCAACGGTATGGACGA AAAAAGGCGCTCAACCTACA 50.0 45.0 57.9 58.0 716-755 bp 1.0 85.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 651-690 bp 1.0 85.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 650-689 bp 1.0 85.3
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 665-704 bp 1.0 84.5
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 660-699 bp 1.0 84.3