Hotspot

trnH-GUG-psbA

LSC · 78-539 · 462 bp

Back to Berberis

Pi 0.0131

MarkerSeek score 57.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 19
pi: 0.0131
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACTGCCTTAACCCA	TGGGCTGATATCATCAACCG	50.0	50.0	57.8	57.5	508-635 bp	1.0	80.4
trnH-GUG-psbA_p2	AATCCACTGCCTTAACCCAC	TGGGCTGATATCATCAACCG	50.0	50.0	57.8	57.5	507-634 bp	1.0	80.4
trnH-GUG-psbA_p3	ACAATCCACTGCCTTAACCC	TGGGCTGATATCATCAACCG	50.0	50.0	57.8	57.5	509-636 bp	1.0	80.4
trnH-GUG-psbA_p4	CAATCCACTGCCTTAACCCA	GGGCTGATATCATCAACCGT	50.0	50.0	57.8	57.4	507-634 bp	1.0	80.4
trnH-GUG-psbA_p5	AATCCACTGCCTTAACCCAC	GGGCTGATATCATCAACCGT	50.0	50.0	57.8	57.4	506-633 bp	1.0	80.4