Hotspot

rps16-trnQ-UUG

LSC · 6271-7238 · 968 bp

Back to Berardia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
968
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1041 bp 1.0 78.3
rps16-trnQ-UUG_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1056 bp 1.0 76.4
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1055 bp 1.0 76.4
rps16-trnQ-UUG_p4 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1112 bp 1.0 75.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1045 bp 1.0 73.5