Hotspot

ycf1

IRb · 112427-114235 · 1809 bp

Back to Bennettiodendron

Pi 0.00221

MarkerSeek score 59.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1809
variable_sites: 4
pi: 0.00221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTCGTTTCTAGTCTATCTGTTTCT	TCTTTGCAGGAGAAGGCATC	36.0	50.0	57.5	57.9	2017-2075 bp	1.0	40.2
ycf1_p2	TGTCGTTTCTAGTCTATCTGT	TCTTTGCAGGAGAAGGCATC	38.1	50.0	53.5	57.9	2017-2075 bp	1.0	40.2
ycf1_p3	TGTCGTTTCTAGTCTATCTGTTTCT	CTTTGCAGGAGAAGGCATCA	36.0	50.0	57.5	58.2	2016-2074 bp	1.0	40.2
ycf1_p4	TGTCGTTTCTAGTCTATCTGTTTCT	AGGCAGTCGCATCTCTTTTT	36.0	45.0	57.5	57.8	1986-2044 bp	1.0	40.2
ycf1_p5	TGTCGTTTCTAGTCTATCTGT	CTTTGCAGGAGAAGGCATCA	38.1	50.0	53.5	58.2	2016-2074 bp	1.0	40.2