Hotspot

trnT-GGU

LSC · 32109-32180 · 72 bp

Back to Bennettiodendron

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label trnT-GGU

Feature Metadata

label_name: trnT-GGU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 1
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU_p1	ATCCGCATGCTGGATCATTT	CCGGGGGAGGTTTATGAAAA	45.0	50.0	58.0	57.5	138 bp	1.0	76.4
trnT-GGU_p2	ATCCGCATGCTGGATCATTT	ATAGAAATACTCCCGGGGGA	45.0	50.0	58.0	56.9	150 bp	1.0	71.6
trnT-GGU_p3	ATCCGCATGCTGGATCATTT	CCGGGGGAGGTTTATGAAAAA	45.0	47.6	58.0	58.2	138 bp	1.0	71.0
trnT-GGU_p4	GCATGCTGGATCATTTCGGA	CCGGGGGAGGTTTATGAAAA	50.0	50.0	58.7	57.5	134 bp	1.0	70.9
trnT-GGU_p5	TCCGCATGCTGGATCATTTC	CCGGGGGAGGTTTATGAAAA	50.0	50.0	58.7	57.5	137 bp	1.0	70.9