Hotspot

rps19

IRa · 158062-158340 · 279 bp

Back to Bennettiodendron

Pi 0
MarkerSeek score 33.80
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRa
length_bp
279
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 TTGATTCTTCGTCGCCGTAG TGAACGATAAGCATCTATATTTTCCT 50.0 30.8 58.1 56.6 425 bp 1.0 40.0
rps19_p2 ATTTGATTCTTCGTCGCCGT TGAACGATAAGCATCTATATTTTCCT 45.0 30.8 58.3 56.6 427 bp 1.0 40.0
rps19_p3 TTTGATTCTTCGTCGCCGTA TGAACGATAAGCATCTATATTTTCCT 45.0 30.8 57.6 56.6 426 bp 1.0 40.0
rps19_p4 TTGATTCTTCGTCGCCGTAG TGAACGATAAGCATCTATATTTTCC 50.0 32.0 58.1 55.4 425 bp 1.0 40.0
rps19_p5 TTGATTCTTCGTCGCCGTAG TGAACGATAAGCATCTATATTTTCCTT 50.0 29.6 58.1 57.2 425 bp 1.0 40.0