Hotspot

rbcL-accD

LSC · 57914-58583 · 670 bp

Back to Bennettiodendron

Pi 0.00299

MarkerSeek score 60.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 2
pi: 0.00299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	CGGGTCTTCACTTACACTGG	50.0	55.0	57.9	57.9	873 bp	1.0	78.5
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	CGGGTCTTCACTTACACTGG	50.0	55.0	57.9	57.9	875 bp	1.0	78.5
rbcL-accD_p3	AATGGAGCCCTGAACTAGCT	CGGGTCTTCACTTACACTGG	50.0	55.0	58.4	57.9	870 bp	1.0	76.3
rbcL-accD_p4	CTAGCAAATGGAGCCCTGAA	CGGGTCTTCACTTACACTGG	50.0	55.0	57.6	57.9	876 bp	1.0	76.1
rbcL-accD_p5	AAATGGAGCCCTGAACTAGC	CGGGTCTTCACTTACACTGG	50.0	55.0	57.6	57.9	871 bp	1.0	76.1