Hotspot

trnL-UAA-trnF-GAA

LSC · 48400-48728 · 329 bp

Back to Bennettiodendron

Pi 0.00929

MarkerSeek score 60.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 329
variable_sites: 3
pi: 0.00929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCTGCTGAGCTATCCC	50.0	55.0	57.5	58.0	383 bp	1.0	78.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	419 bp	1.0	77.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCTG	50.0	55.0	57.5	57.9	395 bp	1.0	76.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	416 bp	1.0	74.3
trnL-UAA-trnF-GAA_p5	TTCAAGTCCCTCTATCCCCA	TCTACCTGCTGAGCTATCCC	50.0	55.0	57.0	58.0	372 bp	1.0	74.2