Hotspot

matK-trnK-UUU

LSC · 3558-4316 · 759 bp

Back to Bennettiodendron

Pi 0.00264

MarkerSeek score 60.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 2
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCCGAAAGATAAGTGGG	TGGGTTGCTAACTCAATGGT	50.0	45.0	56.6	57.0	882-883 bp	1.0	61.4
matK-trnK-UUU_p2	CTCCCGAAAGATAAGTGGGT	TGGGTTGCTAACTCAATGGT	50.0	45.0	56.6	57.0	881-882 bp	1.0	61.4
matK-trnK-UUU_p3	ACTCCCGAAAGATAAGTGGG	TCAATGGTAGAGTACTCGGCT	50.0	47.6	56.6	58.0	870-871 bp	1.0	61.1
matK-trnK-UUU_p4	CTCCCGAAAGATAAGTGGGT	TCAATGGTAGAGTACTCGGCT	50.0	47.6	56.6	58.0	869-870 bp	1.0	61.1
matK-trnK-UUU_p5	ACTCCCGAAAGATAAGTGGGT	TGGGTTGCTAACTCAATGGT	47.6	45.0	58.5	57.0	882-883 bp	1.0	60.8