Hotspot

ycf1

SSC · 123724-129177 · 5454 bp

Back to Bellevalia

Pi 0.0147

MarkerSeek score 58.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5454
variable_sites: 80
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGAGAAAAACCAAGGTTCT	TCGATATTGAGACCTGGGCT	38.1	50.0	55.0	57.9	235-241 bp	1.0	52.4
ycf1_p2	TTCTGAGAAAAACCAAGGTTCT	TCGATATTGAGACCTGGGCT	36.4	50.0	55.8	57.9	236-242 bp	1.0	50.7
ycf1_p3	TCTGAGAAAAACCAAGGTTCT	TATGCATATCGACACCGGGA	38.1	50.0	55.0	58.4	213-219 bp	1.0	50.6
ycf1_p4	TCTGAGAAAAACCAAGGTTCT	GCATATCGACACCGGGATTC	38.1	55.0	55.0	58.6	210-216 bp	1.0	49.2
ycf1_p5	TCTGAGAAAAACCAAGGTTCTAGA	TCGATATTGAGACCTGGGCT	37.5	50.0	57.5	57.9	235-241 bp	1.0	48.7