Hotspot

rpl2-trnH-GUG

IRa · 153903-153949 · 47 bp

Back to Bellevalia

Pi 0.0426

MarkerSeek score 66.00

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 47
variable_sites: 2
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	GATTGTGAATCCACCATGCG	50.0	50.0	58.0	57.5	168 bp	1.0	76.9
rpl2-trnH-GUG_p2	TATCCTGCGCTTGGAAGAAG	GATTGTGAATCCACCATGCG	50.0	50.0	57.7	57.5	166 bp	1.0	74.6
rpl2-trnH-GUG_p3	CTGCGCTTGGAAGAAGAAGT	GATTGTGAATCCACCATGCG	50.0	50.0	58.5	57.5	162 bp	1.0	73.3
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	GGGTTCAATTCCCGTCGTT	50.0	52.6	58.0	58.1	147 bp	1.0	72.4
rpl2-trnH-GUG_p5	GTTATCCTGCGCTTGGAAGA	GGGTTCAATTCCCGTCGTTC	50.0	55.0	58.0	59.2	147 bp	1.0	71.3