Hotspot

ndhE-ndhG

SSC · 117844-118027 · 184 bp

Back to Bellevalia

Pi 0.0217

MarkerSeek score 63.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 184
variable_sites: 4
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGTGCTCTAACCATGTTTCGA	AGGTGCAATTACTATGGCTCG	42.9	47.6	57.3	58.2	304 bp	1.0	58.3
ndhE-ndhG_p2	TGCTCTAACCATGTTTCGACT	AGGTGCAATTACTATGGCTCG	42.9	47.6	57.3	58.2	302 bp	1.0	58.3
ndhE-ndhG_p3	GTGCTCTAACCATGTTTCGAC	AGGTGCAATTACTATGGCTCG	47.6	47.6	57.3	58.2	303 bp	1.0	58.0
ndhE-ndhG_p4	AGTGCTCTAACCATGTTTCGA	GGTGCAATTACTATGGCTCG	42.9	50.0	57.3	56.7	303 bp	1.0	57.0
ndhE-ndhG_p5	TGCTCTAACCATGTTTCGACT	GGTGCAATTACTATGGCTCG	42.9	50.0	57.3	56.7	301 bp	1.0	57.0