Hotspot

trnH-GUG-rpl2

IRb · 84012-84058 · 47 bp

Back to Bellevalia

Pi 0.0426

MarkerSeek score 66.00

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 47
variable_sites: 2
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	GATTGTGAATCCACCATGCG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.5	58.0	168 bp	1.0	76.9
trnH-GUG-rpl2_p2	GATTGTGAATCCACCATGCG	TATCCTGCGCTTGGAAGAAG	50.0	50.0	57.5	57.7	166 bp	1.0	74.6
trnH-GUG-rpl2_p3	GATTGTGAATCCACCATGCG	CTGCGCTTGGAAGAAGAAGT	50.0	50.0	57.5	58.5	162 bp	1.0	73.3
trnH-GUG-rpl2_p4	GGGTTCAATTCCCGTCGTT	GTTATCCTGCGCTTGGAAGA	52.6	50.0	58.1	58.0	147 bp	1.0	72.4
trnH-GUG-rpl2_p5	GGGTTCAATTCCCGTCGTTC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	59.2	58.0	147 bp	1.0	71.3