Hotspot

accD-psaI

LSC · 58570-58941 · 372 bp

Back to Bellevalia

Pi 0.00873

MarkerSeek score 40.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 2
pi: 0.00873
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACTTGGGATCCCATGGATG	AAAGAAGCCATTGCAATCGC	55.0	45.0	58.0	58.0	1000-1143 bp	1.0	85.3
accD-psaI_p2	CACTTGGGATCCCATGGATG	TTGTTTTTCGAGACCCGGTT	55.0	45.0	58.0	58.0	1455-1541 bp	1.0	85.3
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.0	58.0	465-608 bp	1.0	84.7
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.0	58.0	459-602 bp	1.0	84.4
accD-psaI_p5	TGGGATCTGTAGTAGGCGAG	AAAGAAGCCATTGCAATCGC	55.0	45.0	58.0	58.0	943 bp	0.5	59.9